Journal: bioRxiv

Article Title: Hypoadiponectinemia does not enhance anxiety-like behaviour in a lean PCOS-like mouse model

doi: 10.64898/2026.03.13.711682

Figure Lengend Snippet: (A) Schematic representation of the breeding scheme generating wildtype (wt) & adiponectin heterozygous (APNhet) littermates. (B) describes the overall study plan where the effects of adiponectin deficient with and without prenatal androgenization (PNA) on anxiety-like behavior were studied using elevated plus maze and open field followed by dissection. Dihydrotestosterone; DHT (created with https://BioRender.com ).

Article Snippet: Total and high molecular weight (HMW) adiponectin levels were measured by ELISA (80-ADPHU-E01, Alpco, Salem, NH, USA).

Techniques: Dissection

Journal: bioRxiv

Article Title: Hypoadiponectinemia does not enhance anxiety-like behaviour in a lean PCOS-like mouse model

doi: 10.64898/2026.03.13.711682

Figure Lengend Snippet: (A) Total and HMW adiponectin in women with PCOS (n= 179) and controls (n= 228), (B) Correlation between serum adiponectin and mental component summary score and ( C ) between serum adiponectin and free testosterone in women with BMI<30 (n=113). Mann-Whitney U-test was used to compare women with and without PCOS. Correlations were analyzed with Spearman’s rank correlation test. Data presented as mean ± SEM.

Article Snippet: Total and high molecular weight (HMW) adiponectin levels were measured by ELISA (80-ADPHU-E01, Alpco, Salem, NH, USA).

Techniques: MANN-WHITNEY

Journal: bioRxiv

Article Title: Hypoadiponectinemia does not enhance anxiety-like behaviour in a lean PCOS-like mouse model

doi: 10.64898/2026.03.13.711682

Figure Lengend Snippet: (A) Total serum adiponectin in 4-month-old wild-type (wt) and adiponectin heterozygous (APNhet) female mice with and without prenatal androgenization (PNA), ( B) total adiponectin in CSF measured in wild-type (wt) and adiponectin heterozygous (APNhet) female mice. Differences in serum and CSF adiponectin compared to wt veh was analyzed using One-way ANOVA. Data are expressed as mean ± SEM and *P<0.05, **P<0.01, *** P < 0.001

Article Snippet: Total and high molecular weight (HMW) adiponectin levels were measured by ELISA (80-ADPHU-E01, Alpco, Salem, NH, USA).

Techniques:

Journal: bioRxiv

Article Title: Hypoadiponectinemia does not enhance anxiety-like behaviour in a lean PCOS-like mouse model

doi: 10.64898/2026.03.13.711682

Figure Lengend Snippet: (A) Time spent in open arms, (B) Time spent in closed arms, (C) Time spent in centre, and (D) number of entries into closed arms in 4-month-old wild-type (wt) and adiponectin heterozygous (APNhet) female mice with and without prenatal androgenization (PNA). The effect of PNA in wt mice was analysed by an unpaired Student’s t-test. wt PNA mice were compared to APNhet PNA to determine if there was an additive effect of adiponectin deficiency. Data are expressed as mean ± SEM and *P < 0.05, **P < 0.01

Article Snippet: Total and high molecular weight (HMW) adiponectin levels were measured by ELISA (80-ADPHU-E01, Alpco, Salem, NH, USA).

Techniques:

Journal: bioRxiv

Article Title: Hypoadiponectinemia does not enhance anxiety-like behaviour in a lean PCOS-like mouse model

doi: 10.64898/2026.03.13.711682

Figure Lengend Snippet:

Article Snippet: Total and high molecular weight (HMW) adiponectin levels were measured by ELISA (80-ADPHU-E01, Alpco, Salem, NH, USA).

Techniques:

Journal: bioRxiv

Article Title: Hypoadiponectinemia does not enhance anxiety-like behaviour in a lean PCOS-like mouse model

doi: 10.64898/2026.03.13.711682

Figure Lengend Snippet: (A) Quantitative analyses of the mean number of preantral, antral & corpora lutea (CL), n=4/group (B) Serum Anti-mullerian hormone (AMH) levels in 4-month-old wild-type (wt) and adiponectin heterozygous (APNhet) offsprings with and without prenatal androgenization (PNA). The effect of PNA in wt mice was analysed by an unpaired Student’s t-test. wt PNA mice were compared to APNhet PNA to determine if there was an additive effect of adiponectin deficiency. Data are expressed as mean ± SEM and * P < 0.05

Article Snippet: Total and high molecular weight (HMW) adiponectin levels were measured by ELISA (80-ADPHU-E01, Alpco, Salem, NH, USA).

Techniques: